Nanogold in Rapid Tests


  • Žiga Jelen University of Maribor Faculty of Mechanical Engineering, Slovenia; Pomurje Science and Innovation Centre, Dvorec Rakičan, Lendavska ulica 28, Rakičan, 9000 Murska Sobota



Gold nanoparticles (AuNPs) have been successfully used as biosensors since the early 1990s, when researchers first demonstrated the use of AuNPs functionalized with DNA probes as a sensing platform for the detection of specific DNA sequences. Since then, gold nanoparticle-based biosensors have seen rapid advancements and have been applied in various fields, including medical diagnostics, environmental monitoring, and food safety. Where they are commonly applied as point of care rapid tests based on functionalised AuNPs, for use as highly specific, reliable, and simple to use rapid tests for the determination of pregnancy, diseases, allergies, and many others. This short review article offers a quick insight into the different types of rapid tests and their components.


Gold nanoparticles, AuNPs, functionalized, rapid tests, diagnostics
Supporting Agencies
Slovenian Research Agency, Training and funding of a Young Researcher, (Co) financing agreements nos. 1000-19-0552, 1000-20-0552 and 1000-21-0552.


Ahmad, Md Imteyaz, and S. S. Bhattacharya. "Size Effect on the Lattice Parameters of Nanocrystalline Anatase." Applied Physics Letters 95 (19) (2009): 877-78.

Amendola, Vincenzo, Roberto Pilot, Marco Frasconi, Onofrio M. Maragò, and Maria Antonia Iatì. "Surface Plasmon Resonance in Gold Nanoparticles: A Review." Journal of Physics Condensed Matter 29 (20) (2017): 203002.

Bahadır, Elif Burcu, and Mustafa Kemal Sezgintürk. "Lateral Flow Assays: Principles, Designs and Labels." TrAC - Trends in Analytical Chemistry 82 (2016): 286-306.

Balerna, A., E. Bernieri, P. Picozzi, A. Reale, S. Santucci, E. Burattini, and S. Mobilio. "Extended X-ray-absorption fine-structure and near-edge-structure studies on evaporated small clusters of Au." Physical Review B 31, no. 8 (1985): 5058.

Bjerner, Johan, Kari Hauge Olsen, Ole P. Børmer, and Kjell Nustad. "Testing and Validating a Homogeneous Immunometric Assay for Interference." Clinical Chemistry and Laboratory Medicine 42 (2) (2004): 208-14.

Borse, Vivek, and Rohit Srivastava. "Process Parameter Optimization for Lateral Flow Immunosensing." Materials Science for Energy Technologies 2 (3) (2019): 434-41.

Byzova, Nadezhda A., Anatoly V. Zherdev, Boris N. Khlebtsov, Andrey M. Burov, Nikolai G. Khlebtsov, and Boris B. Dzantiev. "Advantages of Highly Spherical Gold Nanoparticles as Labels for Lateral Flow Immunoassay." Sensors (Switzerland) 20 (12) (2020): 1-15.

Chen, He, Qian Yang, Yuan Ding, Natalia Vasylieva, Candace S. Bever, Xiude Hua, Minghua Wang, and Bruce D. Hammock. "Competitive and Noncompetitive Immunoassays for the Detection of Benzothiostrobin Using Magnetic Nanoparticles and Fluorescein Isothiocyanate-Labeled Peptides." Analytical and Bioanalytical Chemistry 411 (2) (2019): 527-35.

Huang, Chao, Tian Wen, Feng Juan Shi, Xiao Yan Zeng, and Yong Jun Jiao. "Rapid Detection of IgM Antibodies against the SARS-CoV-2 Virus via Colloidal Gold Nanoparticle-Based Lateral-Flow Assay." ACS Omega 5 (21) (2020): 12550-56.

Huang, Zaixing, Peter Thomson, and Shenglin Di. "Lattice Contractions of a Nanoparticle Due to the Surface Tension: A Model of Elasticity." Journal of Physics and Chemistry of Solids 68 (4) (2007): 530-35.

Jain, Prashant K., Kyeong Seok Lee, Ivan H. El-Sayed, and Mostafa A. El-Sayed. "Calculated Absorption and Scattering Properties of Gold Nanoparticles of Different Size, Shape, and Composition: Applications in Biological Imaging and Biomedicine." Journal of Physical Chemistry B 110 (14) (2006): 7238-48.

Jazayeri, Mir Hadi, Hamed Amani, Ali Akbar Pourfatollah, Hamidreza Pazoki-Toroudi, and Bijan Sedighimoghaddam. "Various Methods of Gold Nanoparticles (GNPs) Conjugation to Antibodies." Sensing and Bio-Sensing Research 9 (2016): 17-22.

Kelly, K. Lance, Eduardo Coronado, Lin Lin Zhao, and George C. Schatz. "The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment." Childhood Education 85 (5) (2009): 293-99.

Li, Zhengtu, Yongxiang Yi, Xiaomei Luo, Nian Xiong, Yang Liu, Shaoqiang Li, Ruilin Sun, et al. "Development and Clinical Application of a Rapid IgM-IgG Combined Antibody Test for SARS-CoV-2 Infection Diagnosis." Journal of Medical Virology 92 (9) (2020): 1518-24.

Low, Kim Fatt, Abdah Karimah, and Chan Yean Yean. "A Thermostabilized Magnetogenosensing Assay for DNA Sequence-Specific Detection and Quantification of Vibrio Cholerae." Biosensors and Bioelectronics 47 (2013): 38-44.

Merck Millipore. "Rapid Lateral Flow Test Strips Considerations for Product Development." Merck Millipore. Billerica: EMD Millipore Corporation (2013).

Mie, G. "Beiträge Zur Optik Trüber Medien, Speziell Kolloidaler Metallösungen" No. 3 (1908): 69.

Mieszawska, Aneta J., Willem J.M. Mulder, Zahi A. Fayad, and David P. Cormode. "Multifunctional Gold Nanoparticles for Diagnosis and Therapy of Disease." Molecular Pharmaceutics 10 (3) (2013): 831-47.

Mohit, Elham, Zahra Rostami, and Hossein Vahidi. "A Comparative Review of Immunoassays for COVID-19 Detection." Expert Review of Clinical Immunology 0 (0) (2021).

Moitra, Parikshit, Maha Alafeef, Ketan Dighe, Matthew B. Frieman, Dipanjan Pan, and Dipanjan Pan. "Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles." ACS Nano 14 (6) (2020): 7617-27.

Nuccetelli, Marzia, Massimo Pieri, Francesca Gisone, and Sergio Bernardini. "Combined Anti-SARS-CoV-2 IgA, IgG, and IgM Detection as a Better Strategy to Prevent Second Infection Spreading Waves." Immunological Investigations 00 (00) (2020): 1-13.

Parolo, Claudio, Amadeo Sena-Torralba, José Francisco Bergua, Enric Calucho, Celia Fuentes-Chust, Liming Hu, Lourdes Rivas, et al. "Tutorial: Design and Fabrication of Nanoparticle-Based Lateral-Flow Immunoassays." Nature Protocols 15 (12) (2020): 3788-3816.

S. Cavalera, B. Colitti, S. Rosati, G. Ferrara, L. Bertolotti, C. Nogarol, C. Guiotto, C. Cagnazzo, M. Denina, F. Fagiolic, F. Di Nardo, M. Chiarello, C. Baggiani, L. Anfossi. "A Multi-Target Lateral Flow Immunoassay Enabling the Specific and Sensitive Detection of Total Antibodies to SARS COV-2." Talanta 14(4) (January) (2020): 337-39.

Sattarahmady, N., A. Movahedpour, H. Heli, and G. R. Hatam. "Gold Nanoparticles-Based Biosensing of Leishmania Major KDNA Genome: Visual and Spectrophotometric Detections." Sensors and Actuators, B: Chemical 235 (2016): 723-31.

Vashist, Sandeep K., and John H.T. Luong. "Immunoassays: An Overview." Handbook of Immunoassay Technologies: Approaches, Performances, and Applications. Elsevier Inc. (2018).

Vashist SK, et al. "In Vitro Diagnostic Assays for COVID-19 : Recent Advances and Emerging Trends." Diagnostics 10 (2020): 202.

Vollath, Dieter, Franz Dieter Fischer, and David Holec. "Surface Energy of Nanoparticles - Influence of Particle Size and Structure." Beilstein Journal of Nanotechnology 9 (1) (2018): 2265-76.

Vu, Binh V., Dmitri Litvinov, and Richard C. Willson. "Gold Nanoparticle Effects in Polymerase Chain Reaction: Favoring of Smaller Products by Polymerase Adsorption." Analytical Chemistry 80 (14) (2008): 5462-67.

Wen, Tian, Chao Huang, Feng Juan Shi, Xiao Yan Zeng, Tian Lu, Shou Nian Ding, and Yong Jun Jiao. "Development of a Lateral Flow Immunoassay Strip for Rapid Detection of IgG Antibody against SARS-CoV-2 Virus." Analyst 145 (15) (2020): 5345-52.

Wild, David. "How to Use This Book." The Immunoassay Handbook: Theory and Applications of Ligand Binding, ELISA and Related Techniques. Fourth Edi. Elsevier Ltd. (2013).

Wild, David, ed. The immunoassay handbook: theory and applications of ligand binding, ELISA and related techniques. Newnes, 2013.

Yang, Jianyu, Xin Wang, Yuyang Sun, Bo Chen, Fangxin Hu, Chunxian Guo, and Ting Yang. "Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection." Biosensors 13 (1) (2023): 1-22.

Yu, Fei, Lanying Du, David M. Ojcius, Chungen Pan, and Shibo Jiang. "Measures for Diagnosing and Treating Infections by a Novel Coronavirus Responsible for a Pneumonia Outbreak Originating in Wuhan, China." Microbes and Infection 22 (2) (2020): 74-79.

Zeng, Shuwen, Dominique Baillargeat, Ho Pui Ho, and Ken Tye Yong. "Nanomaterials Enhanced Surface Plasmon Resonance for Biological and Chemical Sensing Applications." Chemical Society Reviews 43 (10) (2014): 3426-52.

Zhu, Xiaoli, and Tao Gao. "Spectrometry." Nano-Inspired Biosensors for Protein Assay with Clinical Applications. Elsevier Inc. (2018).





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